Evaluation the enzyme immunosorbent assay IDEIA test detecting Chlamydia trachomatis in cervix.
نویسندگان
چکیده
A total of 87 cervical specimens of unselected female sex workers in massage parlors were tested by an enzyme amplified immunoassay IDEIA Chlamydia test and cell culture for the presence of Chlamydia trachomatis. The prevalence of C. trachomatis was 28 (32%) cases and 34 (39%) cases by the cell culture and the IDEIA Chlamydia test respectively. The IDEIA Chlamydia test demonstrated the sensitivity and specificity of 85.7% and 83% respectively, positive and negative predictive values of 70.5% and 92.4% respectively.
منابع مشابه
Improved sensitivity of an enzyme immunoassay IDEIA for detecting Chlamydia trachomatis.
In tests on 375 genital tract specimens a commercially available enzyme immunoassay for Chlamydia trachomatis (IDEIA; Boots-Celltech) was found to have sensitivity values of 62% for men and 74% for women, and a specificity of 97% for both groups, relative to the results obtained by a fluorescence assay (Micro Trak; Syva). The positive predictive value and the negative predictive value of the im...
متن کاملMolecular Evidence of Chlamydia trachomatis Infection and its Relation to Miscarriage
Objective Chlamydia trachomatis (CT) infection is the most common sexually transmitted disease in the world that can persist and also ascend in the genital tract. This intracellular and silent infection is related to some adverse pregnancy outcomes, such as miscarriage. The aims of this study were to explore the best CT screening tests using blood and vaginal samples and to investigate the corr...
متن کاملEvaluation of a new amplified enzyme immunoassay (EIA) for the detection of Chlamydia trachomatis in male urine, female endocervical swab, and patient obtained vaginal swab specimens.
AIMS To compare the performance of a new generation dual amplified enzyme immunoassay (EIA) with a molecular method for the diagnosis of Chlamydia trachomatis, using a range of urogenital samples, and to assess the reliability of testing self collected vaginal specimens compared with clinician collected vaginal specimens. METHODS Two population groups were tested. For the first population gro...
متن کاملComparison of a polymer conjugate-enhanced enzyme immunoassay to ligase chain reaction for diagnosis of Chlamydia trachomatis in endocervical swabs.
Two endocervical swabs from each of 1,123 women were collected into manufacturer-supplied transport tubes and tested for Chlamydia trachomatis by a polymer conjugate-enhanced (PCE) enzyme immunoassay (EIA) (IDEIA PCE Chlamydia; DAKO) and a ligase chain reaction assay (LCx Chlamydia; Abbott). After confirmation by the EIA blocking test, the sensitivity of the IDEIA PCE remained at 91.8% and the ...
متن کاملDevelopment of ELISAs for the detection of urogenital chlamydia trachomatis infection targeting the pORF5 protein.
OBJECTIVE To prepare antibodies against pORF5 plasmid protein of Chlamydia trachomatis and develop double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) for the detection of genital C. trachomatis infections. METHODS The pORF5 protein was expressed in Escherichia coli and used to immunize BALB/c mice and New Zealand rabbits to produce monoclonal antibodies (mAbs) and polycl...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Southeast Asian journal of tropical medicine and public health
دوره 22 4 شماره
صفحات -
تاریخ انتشار 1991